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FGFBP1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FGFBP1 Polyclonal Antibody AP56797
    Sample:
    A431(Human) Cell Lysate at 30 ug
    Primary: Anti-FGFBP1 (bs-1768R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 28 kD
    Observed band size: 50 kD
    detail
  • IHC-P - FGFBP1 Polyclonal Antibody AP56797
    Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGFBP1) Polyclonal Antibody, Unconjugated (bs-1768R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - FGFBP1 Polyclonal Antibody AP56797
    Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-FGFBP1 Polyclonal Antibody, Unconjugated(bs-1768R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - FGFBP1 Polyclonal Antibody AP56797
    Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-FGFBP1 Polyclonal Antibody, Unconjugated(bs-1768R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, E
Primary Accession Q14512
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 26264 Da
Additional Information
Gene ID 9982
Other Names Fibroblast growth factor-binding protein 1, FGF-BP, FGF-BP1, FGF-binding protein 1, FGFBP-1, 17 kDa heparin-binding growth factor-binding protein, 17 kDa HBGF-binding protein, HBp17, FGFBP1, FGFBP, HBP17
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name FGFBP1
Synonyms FGFBP, HBP17
Function Acts as a carrier protein that release fibroblast-binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth.
Cellular Location Secreted, extracellular space. Cell membrane; Peripheral membrane protein. Note=Extracellular and plasma membrane- associated. Colocalizes with HSPG2 in the pericellular environment of squamous cell carcinomas.
Tissue Location Expressed in the suprabasal region of the epidermis, in hair follicles, the basement membrane at the dermo- epidermal junction (occasionally extending into the basement membrane of dermal blood vessels), wounded skin and several invasive squamous cell carcinomas (at protein level). Expressed in normal and wounded skin and various squamous cell carcinomas
Research Areas
Citations (0)
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$ 350.00
Cat# AP56797
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