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>   home   >   Products   >   Primary Antibodies   >   H4-K20 Antibody (N-term)   

H4-K20 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - H4-K20 Antibody (N-term) AP5660a
    H4-K20 Antibody (N-term) (Cat. #AP5660a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the H4 antibody detected the H4-K20(arrow). From left to right ,the Sample Lot# is SA090513AM?SA090513AN .
    detail
  • WB - H4-K20 Antibody (N-term) AP5660a
    H4-K20 Antibody (N-term) (Cat. #AP5660a) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the H4 antibody detected the H4-K20 (arrow). From left to right ,the Sample Lot# is SA090513AM?SA090513AN .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P62805
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 11367 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names Histone H4, HIST1H4A, H4/A, H4FA
Target/Specificity This H4-K20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H4-K20.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsH4-K20 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H4C1
Synonyms H4/A, H4FA, HIST1H4A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)
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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

References

Yan,D., et.al., Biochem. J. 408 (1), 113-121 (2007)

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$ 365.00
$ 140.00
Cat# AP5660a
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