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XRCC1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC, IHC-P, WB, E |
|---|---|
| Primary Accession | P18887 |
| Other Accession | NP_006288.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 69498 Da |
| Antigen Region | 407-435 aa |
| Gene ID | 7515 |
|---|---|
| Other Names | DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1 |
| Target/Specificity | This XRCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the Central region of human XRCC1. |
| Dilution | IF~~1:25 WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | XRCC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | XRCC1 {ECO:0000303|PubMed:2247054, ECO:0000312|HGNC:HGNC:12828} |
|---|---|
| Function | Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed:11163244, PubMed:28002403). Negatively regulates ADP- ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed:28002403, PubMed:34102106, PubMed:34811483). Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed:14500814, PubMed:34102106, PubMed:34811483). |
| Cellular Location | Nucleus. Chromosome Note=Moves from the nucleoli to the global nuclear chromatin upon DNA damage (PubMed:28002403). Recruited to DNA damage sites fowwing interaction with poly-ADP-ribose chains (PubMed:14500814) |
| Tissue Location | Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
References
Lamerdin, J.E., et al. Genomics 25(2):547-554(1995)
Gyapay, G., et al. Nat. Genet. 7 (2 SPEC NO), 246-339 (1994) :
Thompson, L.H., et al. Mol. Cell. Biol. 10(12):6160-6171(1990)
Thompson, L.H., et al. Genomics 5(4):670-679(1989)
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