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Anti-HSP20 (pS16) Antibody

     
  • WB - Anti-HSP20 (pS16) Antibody AP53928
    Western blot analysis of HSP20 (pS16) expression in H460 (A), rat heart (B) whole cell lysates.
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  •  - Anti-HSP20 (pS16) Antibody AP53928
    Immunohistochemical analysis of HSP20 (pS16) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  • IF - Anti-HSP20 (pS16) Antibody AP53928
    Immunofluorescent analysis of HSP20 (pS16) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession O14558
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 17136 Da
Additional Information
Gene ID 126393
Other Names Heat shock protein beta-6; HspB6; Heat shock 20 kDa-like protein p20
Target/Specificity Recognizes endogenous levels of HSP20 (pS16) protein.
Dilution WB~~1/500 - 1/2000
IF~~1/50 - 1/100
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HSPB6
Function Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding- competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity.
Cellular Location Cytoplasm. Nucleus. Secreted Note=Translocates to nuclear foci during heat shock
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to HSP20 (pS16)

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$ 350.00
Cat# AP53928
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