Anti-ASF1B Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
---|---|
Primary Accession | Q9NVP2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 22434 Da |
Gene ID | 55723 |
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Other Names | Histone chaperone ASF1B; Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-II |
Target/Specificity | Recognizes endogenous levels of ASF1B protein. |
Dilution | WB~~1/500 - 1/1000 |
Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | ASF1B {ECO:0000303|PubMed:11470414, ECO:0000312|HGNC:HGNC:20996} |
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Function | Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251, PubMed:21454524, PubMed:26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed:20953179, PubMed:21454524, PubMed:26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Required for gonad development (PubMed:12842904). |
Cellular Location | Nucleus. Cytoplasm, cytosol |
Tissue Location | Highly expressed in testis and at lower levels in colon, small intestine and thymus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Rabbit polyclonal antibody to ASF1B
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