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Anti-Collagen 18 alpha 1 Antibody

     
  • WB - Anti-Collagen 18 alpha 1 Antibody AP53893
    Western blot analysis of Collagen 18 alpha 1 expression in MCF7 (A) whole cell lysates.
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  •  - Anti-Collagen 18 alpha 1 Antibody AP53893
    Immunohistochemical analysis of Collagen 18 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-Collagen 18 alpha 1 Antibody AP53893
    Immunofluorescent analysis of Collagen 18 alpha 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession P39060
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 178188 Da
Additional Information
Gene ID 80781
Other Names Collagen alpha-1(XVIII) chain
Target/Specificity Recognizes endogenous levels of Collagen 18 alpha 1 protein.
Dilution WB~~1/500 - 1/1000
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name COL18A1 (HGNC:2195)
Function Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Cellular Location Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250|UniProtKB:P39061} [Endostatin]: Secreted. Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Location Detected in placenta (at protein level) (PubMed:32337544). Present in multiple organs with highest levels in liver, lung and kidney.
Research Areas
Citations ( 0 )

Background

Rabbit polyclonal antibody to Collagen 18 alpha 1

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$ 350.00
Cat# AP53893
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