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Anti-MYO1D Antibody

     
  • WB - Anti-MYO1D Antibody AP53856
    Western blot analysis of MYO1D expression in rat brain (A), A2780 (B) whole cell lysates.
    detail
  •  - Anti-MYO1D Antibody AP53856
    Immunohistochemical analysis of MYO1D staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession O94832
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 116202 Da
Additional Information
Gene ID 4642
Other Names KIAA0727; Unconventional myosin-Id
Target/Specificity Recognizes endogenous levels of MYO1D protein.
Dilution WB~~1/500 - 1/1000
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name MYO1D
Synonyms KIAA0727
Function Unconventional myosin that functions as actin-based motor protein with ATPase activity (By similarity). Plays a role in endosomal protein trafficking, and especially in the transfer of cargo proteins from early to recycling endosomes (By similarity). Required for normal planar cell polarity in ciliated tracheal cells, for normal rotational polarity of cilia, and for coordinated, unidirectional ciliary movement in the trachea. Required for normal, polarized cilia organization in brain ependymal epithelial cells (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q63357}. Perikaryon {ECO:0000250|UniProtKB:Q63357}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q63357}. Early endosome {ECO:0000250|UniProtKB:F1PRN2}. Cytoplasm, cell cortex {ECO:0000250|UniProtKB:Q63357}. Note=Colocalizes with the actin cytoskeleton in the cell cortex close to the apical cell membrane Colocalizes with cytoplasmic puncta that are reminiscent of transport vesicles. {ECO:0000250|UniProtKB:Q63357}
Tissue Location Expressed in many tissues. Highest levels in brain, followed by lung and ovary; expression is lowest in spleen
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to MYO1D

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$ 350.00
Cat# AP53856
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