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Anti-NDUFS1 Antibody

     
  • WB - Anti-NDUFS1 Antibody AP53691
    Western blot analysis of NDUFS1 expression in HeLa (A), SP2/0 (B), PC12 (C) whole cell lysates.
    detail
  • IF - Anti-NDUFS1 Antibody AP53691
    Immunofluorescent analysis of NDUFS1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P28331
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 79468 Da
Additional Information
Gene ID 4719
Other Names NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; Complex I-75kD; CI-75kD
Target/Specificity Recognizes endogenous levels of NDUFS1 protein.
Dilution WB~~1/500 - 1/1000
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NDUFS1
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:30879903, PubMed:31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed:31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed:30879903, PubMed:31557978).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P15690}; Matrix side {ECO:0000250|UniProtKB:P15690}
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to NDUFS1

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$ 350.00
Cat# AP53691
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