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Anti-ACTN2 Antibody

     
  • WB - Anti-ACTN2 Antibody AP53654
    Western blot analysis of ACTN2 expression in HeLa (A), mouse kidney (B), H9C2 (C) whole cell lysates.
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  •  - Anti-ACTN2 Antibody AP53654
    Immunohistochemical analysis of ACTN2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-ACTN2 Antibody AP53654
    Immunofluorescent analysis of ACTN2 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession P35609
Other Accession Q08043
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 103854 Da
Additional Information
Gene ID 88
Other Names ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
Target/Specificity Recognizes endogenous levels of ACTN2 protein.
Dilution WB~~1/500 - 1/1000
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ACTN2
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Cellular Location Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle
Tissue Location Expressed in both skeletal and cardiac muscle.
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to ACTN2

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$ 350.00
Cat# AP53654
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