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HIST1H2AK Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HIST1H2AK Antibody (N-term) AP5347A
    HIST1H2AK Antibody (N-term) (Cat. #AP5347a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HIST1H2AK antibody detected the HIST1H2AK protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P0C0S8
Other Accession P02263, Q4FZT6, Q8BFU2, Q7L7L0, P35062, Q64523, Q16777, A1A4R1, Q64522, Q8IUE6, P0CC09, Q6GSS7, Q6FI13, P02262, P22752, P0C0S9, Q8CGP7, Q99878, Q8CGP6, Q96KK5, Q64598, Q8CGP5, P0C170, P20671, P0C169, Q93077, P04908, NP_003501.1, C0HKE1, C0HKE2, C0HKE3, C0
Reactivity Human
Predicted Mouse, Rat, Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14091 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 8329;8330;8332;8336;8969
Other Names Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP
Target/Specificity This HIST1H2AK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2AK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H2AK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2AC11 (HGNC:4737)
Synonyms H2AFP, HIST1H2AG
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)
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Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family.

References

Bergink, S., et al. Genes Dev. 20(10):1343-1352(2006)
Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)
Boyne, M.T. II, et al. J. Proteome Res. 5(2):248-253(2006)

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$ 365.00
$ 140.00
Cat# AP5347A
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