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ECI1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ECI1 Antibody AP53297
    All lanes : Anti-ECI1 Antibody at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: NCI-H1299 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P42126
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 33 KDa
Antigen Region 253-302 aa
Additional Information
Gene ID 1632
Other Names Enoyl-CoA delta isomerase 1, mitochondrial, 5.3.3.8, 3, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ECI1
Synonyms DCI
Function Key enzyme of fatty acid beta-oxidation (Probable). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2- trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (By similarity) (PubMed:7818490). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length (By similarity).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P23965}
Tissue Location Expressed in liver (at protein level).
Research Areas
Citations (0)
citation

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Background

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.

References

Janssen U.,et al.Genomics 23:223-228(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Kilponen J.M.,et al.Biochem. J. 300:1-5(1994).
Takahashi Y.,et al.Biochem. J. 304:849-852(1994).

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$ 350.00
Cat# AP53297
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Availability: 4 weeks
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