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DROSHA Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9NRR4 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 160 KDa |
| Gene ID | 29102 |
|---|---|
| Other Names | Ribonuclease 3, Protein Drosha, Ribonuclease III, RNase III, p241, DROSHA, RN3, RNASE3L, RNASEN |
| Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | DROSHA |
|---|---|
| Synonyms | RN3, RNASE3L, RNASEN |
| Function | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri- miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons (By similarity). |
| Cellular Location | Nucleus. Nucleus, nucleolus. Cytoplasm {ECO:0000250|UniProtKB:Q5HZJ0}. Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. |
| Tissue Location | Ubiquitous.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
References
Wu H.,et al.J. Biol. Chem. 275:36957-36965(2000).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Schmutz J.,et al.Nature 431:268-274(2004).
Gunther M.,et al.Mol. Cell. Biochem. 210:131-142(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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