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Acid Sphingomyelinase Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P17405 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 70 KDa |
| Antigen Region | 421 - 480 aa |
| Gene ID | 6609 |
|---|---|
| Other Names | Sphingomyelin phosphodiesterase, Acid sphingomyelinase, aSMase, SMPD1, ASM |
| Target/Specificity | KLH conjugated synthetic peptide derived from human Acid Sphingomyelinase |
| Dilution | WB~~ 1:1000 |
| Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | SMPD1 (HGNC:11120) |
|---|---|
| Function | Converts sphingomyelin to ceramide (PubMed:12563314, PubMed:1840600, PubMed:18815062, PubMed:25339683, PubMed:25920558, PubMed:27659707, PubMed:33163980). Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly (PubMed:20807762, PubMed:21098024, PubMed:9660788). However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form (PubMed:12563314, PubMed:20530211, PubMed:20807762, PubMed:22573858, PubMed:9393854). |
| Cellular Location | Lysosome. Lipid droplet. Secreted. Note=The secreted form is induced in a time- and dose-dependent by IL1B and TNF as well as stress and viral infection. This increase of the secreted form seems to be due to exocytosis of the lysosomal form and is Ca(2+)-dependent (PubMed:20530211, PubMed:20807762, PubMed:22573858). Secretion is dependent of phosphorylation at Ser-510 (PubMed:17303575). Secretion is induced by inflammatory mediators such as IL1B, IFNG or TNF as well as infection with bacteria and viruses (PubMed:12563314, PubMed:20807762) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity.
References
Schuchman E.H.,et al.J. Biol. Chem. 266:8531-8539(1991).
Newrzella D.,et al.Biol. Chem. Hoppe-Seyler 373:1233-1238(1992).
Schuchman E.H.,et al.Genomics 12:197-205(1992).
Ida H.,et al.J. Biochem. 114:15-20(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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