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ENT1 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q99808 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 55 KDa |
| Antigen Region | 1 - 60 aa |
| Gene ID | 2030 |
|---|---|
| Other Names | Equilibrative nucleoside transporter 1, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Equilibrative NBMPR-sensitive nucleoside transporter, Nucleoside transporter, es-type, Solute carrier family 29 member 1, SLC29A1, ENT1 |
| Target/Specificity | KLH conjugated synthetic peptide derived from human ENT1 |
| Dilution | WB~~ 1:1000 |
| Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | SLC29A1 (HGNC:11003) |
|---|---|
| Synonyms | ENT1 |
| Function | Uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed:10722669, PubMed:10755314, PubMed:12527552, PubMed:14759222, PubMed:15037197, PubMed:17379602, PubMed:21795683, PubMed:26406980, PubMed:27995448, PubMed:35790189, PubMed:8986748). Functions as a Na(+)-independent transporter (PubMed:8986748). Involved in the transport of nucleosides such as adenosine, guanosine, inosine, uridine, thymidine and cytidine (PubMed:10722669, PubMed:10755314, PubMed:12527552, PubMed:14759222, PubMed:15037197, PubMed:17379602, PubMed:26406980, PubMed:8986748). Also transports purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed:21795683, PubMed:27995448). Mediates basolateral nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis barrier (By similarity). Regulates inosine levels in brown adipocytes tissues (BAT) and extracellular inosine levels, which controls BAT-dependent energy expenditure (PubMed:35790189). |
| Cellular Location | Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Localized to the basolateral membrane of Sertoli cells (PubMed:23639800). Localized to the cell membrane of erythrocytes (PubMed:11584005, PubMed:23219802). |
| Tissue Location | Expressed in testis at the blood-testis barrier (at protein level) (PubMed:23639800). Detected in erythrocytes (at protein level) (PubMed:11584005, PubMed:23219802). Expressed at relatively high levels in cerebral cortex, particularly the frontal and parietal lobes, and the thalamus and basal ganglia (at protein level) (PubMed:11311901). In the midbrain expressed at moderate levels, whereas in the other areas of the brainstem, namely medulla and pons, cerebellum and the hippocampus expressed at lower amounts when compared to the other brain regions (at protein level) (PubMed:11311901) Expressed in Langerhans cells and lymphocytes in the pancreas (at protein level) (PubMed:15501974). Expressed in kidney, in polarized renal epithelial cells (PubMed:12527552). Expressed in adipose tissues (PubMed:35790189). Expressed in placenta (PubMed:8986748). Expressed in small intestine (PubMed:10755314). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
References
Griffiths M.,et al.Nat. Med. 3:89-93(1997).
Graham K.A.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Lum P.Y.,et al.Cancer Chemother. Pharmacol. 45:273-278(2000).
Sankar N.,et al.Nucleic Acids Res. 30:4339-4350(2002).
Mangravite L.M.,et al.Am. J. Physiol. 284:F902-F910(2003).
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