HSD17B2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | P37059 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 42785 Da |
Antigen Region | 265-294 aa |
Gene ID | 3294 |
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Other Names | Estradiol 17-beta-dehydrogenase 2, 17-beta-hydroxysteroid dehydrogenase type 2, 17-beta-HSD 2, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, Microsomal 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase, HSD17B2, EDH17B2 |
Target/Specificity | This HSD17B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human HSD17B2. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HSD17B2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HSD17B2 (HGNC:5211) |
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Synonyms | EDH17B2, SDR9C2 |
Function | Catalyzes the NAD-dependent oxidation of the highly active 17beta-hydroxysteroids, such as estradiol (E2), testosterone (T), and dihydrotestosterone (DHT), to their less active forms and thus regulates the biological potency of these steroids. Oxidizes estradiol to estrone, testosterone to androstenedione, and dihydrotestosterone to 5alpha-androstan-3,17-dione. Also has 20-alpha-HSD activity. |
Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein |
Tissue Location | Expressed in placenta. |
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Provided below are standard protocols that you may find useful for product applications.
Background
HSD17B2 is capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone.HSD17B2 also has 20-alpha-HSD activity. HSD17B2 uses NADH while EDH17B3 uses NADPH.
References
Shen, Z., et al. Endocrinology 150(11):4941-4949(2009)
Bhavani, V., et al. Cancer Biomark 5(4):207-213(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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