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Glutamine Synthetase Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P15104 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 49 KDa |
| Antigen Region | 201 - 260 aa |
| Gene ID | 2752 |
|---|---|
| Other Names | Glutamine synthetase, GS, Glutamate decarboxylase, Glutamate--ammonia ligase, GLUL, GLNS |
| Target/Specificity | KLH conjugated synthetic peptide derived from human Glutamine Synthetase |
| Dilution | WB~~ 1:4000 |
| Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | GLUL {ECO:0000303|PubMed:30158707, ECO:0000312|HGNC:HGNC:4341} |
|---|---|
| Function | Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:16267323, PubMed:30158707, PubMed:36289327). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed:18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed:36289327). |
| Cellular Location | Cytoplasm, cytosol. Microsome {ECO:0000250|UniProtKB:P09606} Mitochondrion {ECO:0000250|UniProtKB:P09606}. Cell membrane; Lipid-anchor. Note=Mainly localizes in the cytosol, with a fraction associated with the cell membrane |
| Tissue Location | Expressed in endothelial cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
References
Gibbs C.S.,et al.Nucleic Acids Res. 15:6293-6293(1987).
van den Hoff M.J.B.,et al.Biochim. Biophys. Acta 1090:249-251(1991).
Christa L.,et al.Gastroenterology 106:1312-1320(1994).
Haberle J.,et al.Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
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