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ATP6V0A1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q93050 |
| Other Accession | Q8AVM5, P25286, Q9Z1G4, Q9I8D0, Q29466 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse, Rat, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 96413 Da |
| Antigen Region | 44-71 aa |
| Gene ID | 535 |
|---|---|
| Other Names | V-type proton ATPase 116 kDa subunit a isoform 1, V-ATPase 116 kDa isoform a1, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1, ATP6V0A1, ATP6N1, ATP6N1A, VPP1 |
| Target/Specificity | This ATP6V0A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the N-terminal region of human ATP6V0A1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ATP6V0A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATP6V0A1 |
|---|---|
| Synonyms | ATP6N1, ATP6N1A, VPP1 |
| Function | Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that transports protons across cellular membranes. V-ATPase is responsible for the acidification of various organelles, such as lysosomes, endosomes, the trans-Golgi network, and secretory granules, including synaptic vesicles (PubMed:33065002, PubMed:33833240, PubMed:34909687). In certain cell types, can be exported to the plasma membrane, where it is involved in the acidification of the extracellular environment (By similarity). Required for assembly and activity of the vacuolar ATPase (By similarity). Through its action on compartment acidification, plays an essential role in neuronal development in terms of integrity and connectivity of neurons (PubMed:33833240). |
| Cellular Location | Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250|UniProtKB:P25286}; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:P25286}; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles.
References
Antonacopoulou, A.G., et al. Anticancer Res. 28 (2B), 1221-1227 (2008)
Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
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