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Alpha smooth muscle Actin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Alpha smooth muscle Actin Antibody AP50856
    Human A549 lysates probed with Rabbit Anti-Actin Polyclonal Antibody, Unconjugated (AP50856) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
    detail
  • WB - Alpha smooth muscle Actin Antibody AP50856
    Human A549 lysates probed with Rabbit Anti-Actin Polyclonal Antibody, Unconjugated AP50856 at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, E
Primary Accession P62736
Reactivity Human, Mouse, Rat, Rabbit
Host Rabbit
Clonality Polyclonal
Calculated MW 42; [uniprot]42; [CST]45; [EPI]45,42; KDa
Antigen Region 165-260/377 aa
Additional Information
Gene ID 59
Other Names Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS
Dilution WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1 µg/Test,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ACTA2
Synonyms ACTSA, ACTVS
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)
citation

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Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

References

Kamada S.,et al.Nucleic Acids Res. 17:1767-1767(1989).
Reddy S.,et al.J. Biol. Chem. 265:1683-1687(1990).
Kim J.W.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 385.00
Cat# AP50856
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Availability: 4 weeks
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