Cytochrome P450 39A1 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9NYL5 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 54 KDa |
Antigen Region | 375-402 aa |
Gene ID | 51302 |
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Other Names | 24-hydroxycholesterol 7-alpha-hydroxylase, Cytochrome P450 39A1, hCYP39A1, Oxysterol 7-alpha-hydroxylase, CYP39A1 |
Dilution | WB~~ 1:1000 |
Format | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol. |
Storage Conditions | -20℃ |
Name | CYP39A1 {ECO:0000303|PubMed:25201972, ECO:0000312|HGNC:HGNC:17449} |
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Function | A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis (PubMed:10748047, PubMed:25201972). Catalyzes 7-alpha hydroxylation of (24S)- hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver (PubMed:10748047, PubMed:25201972). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:10748047, PubMed:25201972). |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q64654}; Multi-pass membrane protein. Microsome membrane {ECO:0000250|UniProtKB:Q64654}; Multi-pass membrane protein |
Tissue Location | Liver specific.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha- hydroxylated product.
References
Li-Hawkins J.,et al.J. Biol. Chem. 275:16543-16549(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
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