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CHRNA3 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P32297 |
| Other Accession | Q07263 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57480 Da |
| Antigen Region | 25-52 aa |
| Gene ID | 1136 |
|---|---|
| Other Names | Neuronal acetylcholine receptor subunit alpha-3, CHRNA3, NACHRA3 |
| Target/Specificity | This CHRNA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-52 amino acids from the N-terminal region of human CHRNA3. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CHRNA3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CHRNA3 |
|---|---|
| Synonyms | NACHRA3 |
| Function | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. |
| Cellular Location | Postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum {ECO:0000250|UniProtKB:P04757} Golgi apparatus {ECO:0000250|UniProtKB:P04757}. Note=Interaction with UBXN2A/UBXD4 promotes translocation to the plasma membrane {ECO:0000250|UniProtKB:P04757} |
Provided below are standard protocols that you may find useful for product applications.
Background
CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
References
Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010)
Girard, N., et al. Clin. Cancer Res. 16(2):755-763(2010)
Grando, S.A., et al. J. Invest. Dermatol. 105(6):774-781(1995)
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