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ADD1 (Ab-726) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ADD1 (Ab-726) Antibody AP50046
    Immunofluorescence analysis of HeLa cells, using ADD1 (Ab-726) antibody .
    detail
  • WB - ADD1 (Ab-726) Antibody AP50046
    Western blot analysis of lysates from Hela cell line ,using ADD1 (Ab-726) Antibody(B0002). B0002 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug.
    detail
  • IHC - ADD1 (Ab-726) Antibody AP50046
    Immunohistochemical analysis of paraffin-embedded human brain tissue using ADD1 (Ab-726) antibody .
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession P35611
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 81,70,84 KDa
Antigen Region 705-734 aa
Additional Information
Gene ID 118
Other Names Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA
Dilution IF~~1:100
WB~~ 1:1000
IHC~~1:50-1:100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name ADD1
Synonyms ADDA
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Cellular Location Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit
Research Areas
Citations (0)
citation

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Background

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

References

Joshi R.L.,et al.J. Cell Biol. 115:665-675(1991).
Lin B.,et al.Genomics 25:93-99(1995).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Taylor S.A.,et al.Nat. Genet. 2:223-227(1992).

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$ 350.00
Cat# AP50046
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Availability: 4 weeks
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