Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ITPA Antibody (N-term)   

ITPA Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
crown
Products labeled with a "crown" meet aggressive quality standards. Learn more.
  • IF - ITPA Antibody (N-term) AP4942A
    Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term)(Cat. #AP4942a). AP4942a was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
    detail
  • WB - ITPA Antibody (N-term) AP4942A
    Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate(from left to right), using ITPA Antibody (N-term)(Cat. #AP4942a). AP4942a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • IHC-P - ITPA Antibody (N-term) AP4942A
    ITPA Antibody (N-term) (Cat. #AP4942a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITPA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ITPA Antibody (N-term) AP4942A
    ITPA Antibody (N-term) (Cat. #AP4942a) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, IHC-P, E
Primary Accession Q9BY32
Other Accession Q2NLA8, D3ZW55, Q9D892, F1NLH9, Q2KIC5
Reactivity Human
Predicted Bovine, Chicken, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 21446 Da
Antigen Region 24-51 aa
Additional Information
Gene ID 3704
Other Names Inosine triphosphate pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, ITPase {ECO:0000255|HAMAP-Rule:MF_03148}, Inosine triphosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, 36119 {ECO:0000255|HAMAP-Rule:MF_03148}, Non-canonical purine NTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, Non-standard purine NTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, Nucleoside-triphosphate diphosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, Nucleoside-triphosphate pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}, NTPase {ECO:0000255|HAMAP-Rule:MF_03148}, Putative oncogene protein hlc14-06-p, ITPA {ECO:0000255|HAMAP-Rule:MF_03148}, C20orf37
Target/Specificity This ITPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ITPA.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsITPA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ITPA {ECO:0000255|HAMAP-Rule:MF_03148}
Synonyms C20orf37
Function Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03148, ECO:0000269|PubMed:11278832}
Tissue Location Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Background

ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.

References

Fellay, J., et al. Nature 464(7287):405-408(2010)
Herting, G., et al. Biochim. Biophys. Acta 1802(2):269-274(2010)
Kudo, M., et al. Drug Metab. Pharmacokinet. 24(6):557-564(2009)

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 365.00
$ 140.00
Cat# AP4942A
Size:
Quantity:
Availability: In Stock
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"