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FCGR1A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FCGR1A Antibody (Center) AP4930C
    Western blot analysis of FCGR1A Antibody (Center) (Cat. #AP4930c) in NCI-H460 cell line lysates (35ug/lane). FCGR1A (arrow) was detected using the purified Pab.
    detail
  • IHC-P - FCGR1A Antibody (Center) AP4930C
    FCGR1A Antibody (Center) (Cat. #AP4930c) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FCGR1A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - FCGR1A Antibody (Center) AP4930C
    FCGR1A Antibody (Center) (Cat. #AP4930c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P12314
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42632 Da
Antigen Region 217-245 aa
Additional Information
Gene ID 2209
Other Names High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64, FCGR1A, FCG1, FCGR1, IGFR1
Target/Specificity This FCGR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-245 amino acids from the Central region of human FCGR1A.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFCGR1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FCGR1A
Synonyms FCG1, FCGR1, IGFR1
Function High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.
Cellular Location Cell membrane; Single-pass type I membrane protein Note=Stabilized at the cell membrane through interaction with FCER1G
Tissue Location Monocyte/macrophage specific.
Research Areas
Citations (0)
citation

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Background

FCGR1A encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.

References

Poon, I.K., et al. Blood 115(12):2473-2482(2010)
Jung, S.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(2):604-609(2010)
Hulse, K.E., et al. J. Allergy Clin. Immunol. 125(1):247-256(2010)

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$ 365.00
$ 140.00
Cat# AP4930C
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