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ZNF9 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ZNF9 Antibody (Center) AP4784c
    Western blot analysis of ZNF9 Antibody (Center) (Cat. #AP4784c) in Neuro-2a cell line lysates (35ug/lane). ZNF9 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ZNF9 Antibody (Center) AP4784c
    ZNF9 Antibody (Center) (Cat. #AP4784c) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P62633
Other Accession P62634, P53996, O42395, Q3T0Q6
Reactivity Human
Predicted Bovine, Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19463 Da
Antigen Region 67-96 aa
Additional Information
Gene ID 7555
Other Names Cellular nucleic acid-binding protein, CNBP, Zinc finger protein 9, CNBP, RNF163, ZNF9
Target/Specificity This ZNF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-96 amino acids from the Central region of human ZNF9.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZNF9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNBP (HGNC:13164)
Synonyms RNF163, ZNF9
Function Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression (PubMed:2562787). Has a role as transactivator of the Myc promoter (By similarity). Binds single-stranded RNA in a sequence-specific manner (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P53996}. Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:P53996} [Isoform 2]: Cytoplasm [Isoform 5]: Cytoplasm [Isoform 8]: Cytoplasm
Tissue Location Expressed in the liver, kidney, spleen, testis, lung, muscle and adrenal glands.
Citations (0)
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Background

ZNF9 encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2.

References

Lucchiari, S., et al. J. Neurol. Sci. 275 (1-2), 159-163 (2008)
Auvinen, S., et al. Arthritis Rheum. 58(11):3627-3631(2008)
Gerbasi, V.R., et al. Mol. Cell Proteomics 6(6):1049-1058(2007)
Toth, C., et al. Muscle Nerve 35(2):259-264(2007)

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$ 365.00
$ 140.00
Cat# AP4784c
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