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Phospho-eEF2k (Ser366) Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O00418 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 82144 Da |
| Gene ID | 29904 |
|---|---|
| Other Names | Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, 2.7.11.20, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K |
| Target/Specificity | This Phospho-eEF2k (Ser366) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 337-371 amino acids from the human region of human EEF2k. |
| Dilution | WB~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-eEF2k (Ser366) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EEF2K |
|---|---|
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
References
Ryazanov A.G.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889(1997).
Martin J.,et al.Nature 432:988-994(2004).
Pavur K.S.,et al.Biochemistry 39:12216-12224(2000).
Knebel A.,et al.EMBO J. 20:4360-4369(2001).
Wang X.,et al.EMBO J. 20:4370-4379(2001).
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