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Phospho-CCNB1(S35) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Phospho-CCNB1(S35) Antibody AP3882a
    Western blot analysis of lysate from Hela cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody (Cat. #AP3882a) or CCNB1-S9 Antibody.Lysate at 15ug per lane.AP3882a was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
    detail
  • DB - Phospho-CCNB1(S35) Antibody AP3882a
    Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab (Cat. #AP3882a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, DB, E
Primary Accession P14635
Other Accession NP_114172.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48337 Da
Additional Information
Gene ID 891
Other Names G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Target/Specificity This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.
Dilution WB~~1:1100
DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CCNB1(S35) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNB1
Synonyms CCNB
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].

References

Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)
van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)
Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Nantajit, D., et al. PLoS ONE 5 (8), E12341 (2010) :

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$ 365.00
$ 140.00
Cat# AP3882a
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