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phospho-Sox2(S246) Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| DB, IF, E |
|---|---|
| Primary Accession | P48431 |
| Other Accession | P48432, NP_003097.1, P54231 |
| Reactivity | Human |
| Predicted | Mouse, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 34310 Da |
| Gene ID | 6657 |
|---|---|
| Other Names | Transcription factor SOX-2, SOX2 |
| Target/Specificity | This Sox2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S246 of human Sox2. |
| Dilution | IF~~1:200 DB~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | phospho-Sox2(S246) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SOX2 |
|---|---|
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity). |
| Cellular Location | Nucleus speckle {ECO:0000250|UniProtKB:Q05066}. Cytoplasm {ECO:0000250|UniProtKB:Q05738}. Nucleus {ECO:0000250|UniProtKB:Q05738}. Note=Acetylation contributes to its nuclear localization and deacetylation by HDAC3 induces a cytoplasmic delocalization (By similarity). Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH) (By similarity) Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus (By similarity). Nuclear import is facilitated by XPO4, a protein that usually acts as a nuclear export signal receptor (By similarity) {ECO:0000250|UniProtKB:Q05066, ECO:0000250|UniProtKB:Q05738} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
References
References for protein:
1.Gen, Y., et al. Cancer Genet. Cytogenet. 202(2):82-93(2010)
2.Ji, J., et al. Hum. Pathol. 41(10):1438-1447(2010)
3.Fang, X., et al. OMICS 14(4):369-384(2010)
4.Sholl, L.M., et al. Am. J. Surg. Pathol. 34(8):1193-1198(2010)
5.Zhang, D., et al. BMC Med. Genet. 11, 116 (2010)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
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