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Phospho-NFKBIA(Ser32)) Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| DB, E |
|---|---|
| Primary Accession | P25963 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35609 Da |
| Gene ID | 4792 |
|---|---|
| Other Names | NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI |
| Target/Specificity | This NFKBIA Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser32 of human NFKBIA. |
| Dilution | DB~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-NFKBIA(Ser32)) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NFKBIA |
|---|---|
| Synonyms | IKBA, MAD3, NFKBI |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466). |
| Cellular Location | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
- Primary Antibodies
- Cancer
- Cell Biology
- Immunology
- Signal Transduction
- Antibody Collections
- Phospho Antibodies
- Apoptosis signaling
- Apoptosis
- Inflammation mediated by chemokine and cytokine signaling
- Chemokine signaling
- T cell receptor signaling
- T cell activation
- B cell receptor signaling
- Toll-Like receptor signaling
- Toll-Like Receptor
- Toll receptor signaling
- RIG-l-like Receptor
- Host-virus interaction
- Viral process
Provided below are standard protocols that you may find useful for product applications.
Background
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
References
Wu, S., et al. J. Clin. Endocrinol. Metab. 95(3):1220-1228(2010)
Senol Tuncay, S., et al. Biochem. Genet. 48 (1-2), 104-112 (2010)
Hung, Y.H., et al. Dis. Markers 28(1):55-62(2010)
McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
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