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> home > Products > Primary Antibodies > Antibody Collections > Phospho Antibodies > Phospho-eNos(S1177) Antibody
Phospho-eNos(S1177) Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IF, DB, E |
|---|---|
| Primary Accession | P29474 |
| Other Accession | Q62600, Q28969, P70313, P29473, P79209 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Pig, Rat, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 133275 Da |
| Gene ID | 4846 |
|---|---|
| Other Names | Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3 |
| Target/Specificity | This eNos Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1177 of human eNos. |
| Dilution | IF~~1:10~50 DB~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-eNos(S1177) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NOS3 (HGNC:7876) |
|---|---|
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. |
| Cellular Location | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity |
| Tissue Location | Platelets, placenta, liver and kidney. |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.
References
Greif,D.M., et.al., Biochemistry 41 (52), 15845-15853 (2002)
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$ 365.00
$ 140.00
Cat# AP3665a
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