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> home > Products > Primary Antibodies > Antibody Collections > Phospho Antibodies > Bi-Phospho-ERK1/2(T202/Y204) Antibody
Bi-Phospho-ERK1/2(T202/Y204) Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| DB, WB, E |
|---|---|
| Primary Accession | P27361 |
| Other Accession | P21708, Q63844, P40417 |
| Reactivity | Human |
| Predicted | Drosophila, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 43136 Da |
| Gene ID | 5595 |
|---|---|
| Other Names | Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3 |
| Target/Specificity | This ERK1/2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T202/Y204 of human ERK1/2. |
| Dilution | WB~~1:1000 DB~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Bi-Phospho-ERK1/2(T202/Y204) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAPK3 |
|---|---|
| Synonyms | ERK1, PRKM3 |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed:35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P21708}. Nucleus. Membrane, caveola {ECO:0000250|UniProtKB:P21708}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q63844} Note=Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). {ECO:0000250|UniProtKB:Q63844, ECO:0000269|PubMed:19060905} |
Research Areas
- Primary Antibodies
- Cancer
- Cell Biology
- Immunology
- Neuroscience
- Signal Transduction
- Antibody Collections
- Phospho Antibodies
- Apoptosis signaling
- Interleukin signaling
- Interferon-gamma signaling
- Inflammation mediated by chemokine and cytokine signaling
- Chemokine signaling
- T cell receptor signaling
- T cell activation
- Fc gamma R-mediated phagocytosis
- B cell receptor signaling
- Toll-Like receptor signaling
- Toll receptor signaling
- Host-virus interaction
- Viral process
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
References
Munshi, H.G., et al., J. Biol. Chem. 279(37):39042-39050 (2004).
Mukherjee, S., et al., Infect. Immun. 72(9):5274-5282 (2004).
Sebkova, L., et al., Infect. Immun. 72(9):5019-5026 (2004).
Huang, H.M., et al., Biochem. Biophys. Res. Commun. 320(4):1247-1252 (2004).
Mizuno, S., et al., Am. J. Respir. Cell Mol. Biol. 31(2):184-192 (2004).
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$ 365.00
$ 140.00
Cat# AP3607a
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