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Bi-Phospho-YES1(Y537)/Phospho-SRC(Y530) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Bi-Phospho-YES1(Y537)/Phospho-SRC(Y530) Antibody AP3254a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
    detail
  • DB - Bi-Phospho-YES1(Y537)/Phospho-SRC(Y530) Antibody AP3254a
    Dot blot analysis of anti-Phospho-YES1-Y537/Phospho-SRC-Y530 Antibody (Cat.#AP3254a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, DB, E
Primary Accession P12931, P07947
Other Accession Q9WUD9, P05480, Q1JPZ3, P00523, P12931
Reactivity Human
Predicted Chicken, Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Other Names Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src, SRC, SRC1
Target/Specificity This SRC Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y530 of human SRC.
Dilution IHC-P~~1:50~100
DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBi-Phospho-YES1(Y537)/Phospho-SRC(Y530) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations ( 0 )

Background

This protein has tyrosine kinase activity and belongs to the src family of proteins. The YES1 gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene.

References

Anneren, C., et al., J. Biol. Chem. 279(30):31590-31598 (2004).
Reinehr, R., et al., J. Biol. Chem. 279(23):23977-23987 (2004).
Leclerc, P., et al., Biol. Reprod. 67(1):301-307 (2002).
Oh, H.S., et al., J. Biol. Chem. 277(39):36489-36498 (2002).
Silverman, G.A., et al., Genomics 15(2):442-445 (1993).

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$ 365.00
$ 140.00
Cat# AP3254a
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