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GMPS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GMPS Antibody (C-term) AP2953b
    Western blot analysis of GMPS (arrow) using rabbit polyclonal GMPS Antibody (C-term) (Cat. #AP2953b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GMPS gene (Lane 2) (Origene Technologies).
    detail
  • IHC-P - GMPS Antibody (C-term) AP2953b
    Formalin-fixed and paraffin-embedded kidney tissue reacted with GMPS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GMPS Antibody (C-term) AP2953b
    Flow cytometric analysis of hela cells using GMPS Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P49915
Other Accession Q4V7C6, Q3THK7
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 76715 Da
Antigen Region 602-630 aa
Additional Information
Gene ID 8833
Other Names GMP synthase [glutamine-hydrolyzing], GMP synthetase, Glutamine amidotransferase, GMPS
Target/Specificity This GMPS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 602-630 amino acids from the C-terminal region of human GMPS.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGMPS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GMPS
Function Catalyzes the conversion of xanthine monophosphate (XMP) to GMP in the presence of glutamine and ATP through an adenyl-XMP intermediate.
Cellular Location Cytoplasm, cytosol.
Research Areas
Citations (0)
citation

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Background

GMPS is involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.

References

Nakamura,J., et.al., J. Biol. Chem. 270 (40), 23450-23455 (1995)
Nakamura,J. et.al., J. Biol. Chem. 270 (13), 7347-7353 (1995)

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$ 182.50
$ 70.00
Cat# AP2953b
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