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TUFM Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TUFM Antibody (N-term) AP2918a
    Western blot analysis of TUFM Antibody (N-term) (Cat. #AP2918a) in Ramos, Hela cell line lysates (35ug/lane). TUFM (arrow) was detected using the purified Pab.
    detail
  • WB - TUFM Antibody (N-term) AP2918a
    Western blot analysis of TUFM (arrow) using rabbit polyclonal TUFM Antibody (N-term) (Cat. #AP2918a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TUFM gene.
    detail
  • IHC-P - TUFM Antibody (N-term) AP2918a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TUFM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - TUFM Antibody (N-term) AP2918a
    TUFM Antibody (N-term) (Cat.#AP2918a) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P49411
Other Accession P85834, Q8BFR5, P49410
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49875 Da
Antigen Region 75-104 aa
Additional Information
Gene ID 7284
Other Names Elongation factor Tu, mitochondrial, EF-Tu, P43, TUFM
Target/Specificity This TUFM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the N-terminal region of human TUFM.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUFM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUFM
Function Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.
Cellular Location Mitochondrion.
Research Areas
Citations ( 0 )

Background

TUFM is a protein which participates in protein translation in mitochondria. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

References

Valente,L., et.al., Biochim. Biophys. Acta 1792 (8), 791-795 (2009)
Bogenhagen,D.F., et.al., J. Biol. Chem. 283 (6), 3665-3675 (2008)

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$ 182.50
$ 70.00
Cat# AP2918a
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