SSB Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, IHC-P, E |
---|---|
Primary Accession | P05455 |
Other Accession | P10881 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 46837 Da |
Antigen Region | 66-96 aa |
Gene ID | 6741 |
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Other Names | Lupus La protein, La autoantigen, La ribonucleoprotein, Sjoegren syndrome type B antigen, SS-B, SSB |
Target/Specificity | This SSB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SSB. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SSB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SSB |
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Function | Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed:2470590, PubMed:3192525). In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES- mediated translation (PubMed:12384597). |
Cellular Location | Nucleus. |
Provided below are standard protocols that you may find useful for product applications.
Background
SSB is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III -transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. This protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus.
References
Bitko,V., J. Virol. 82 (16), 7977-7987 (2008) Kotik-Kogan,O., Structure 16 (6), 852-862 (2008) Biswas,D., Clin. Rheumatol. 27 (6), 717-722 (2008)
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