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NSE Antibody (Y236)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NSE Antibody (Y236) AP2780a
    Western blot analysis of anti-NSE Antibody (Y236) (Cat.#AP2780a) in Y79 cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab.
    detail
  • IHC-P - NSE Antibody (Y236) AP2780a
    NSE Antibody (Y236) (Cat. #AP2780a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSE Antibody (Y236) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P09104
Other Accession P07323, P17183, NP_001966
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47269 Da
Antigen Region 213-239 aa
Additional Information
Gene ID 2026
Other Names Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Target/Specificity This NSE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-239 amino acids from human NSE.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNSE Antibody (Y236) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO2
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium- dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Cellular Location Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas
Citations (0)
citation

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Background

NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

References

Kotaska,K., Neuro Endocrinol. Lett. 28 (6), 761-764 (2007)
Forooghian,F., J. Clin. Immunol. 27 (4), 388-396 (2007)
Rech,T.H., Crit Care 10 (5), R133 (2006)
Oliva,D., Genomics 10 (1), 157-165 (1991)

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$ 365.00
$ 140.00
Cat# AP2780a
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