PHB Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, IF, FC, E |
---|---|
Primary Accession | P35232 |
Other Accession | P84173, Q3T165 |
Reactivity | Human |
Predicted | Bovine, Chicken |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 29804 Da |
Antigen Region | 89-117 aa |
Gene ID | 5245 |
---|---|
Other Names | Prohibitin, PHB |
Target/Specificity | This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB. |
Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PHB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PHB1 {ECO:0000303|PubMed:28017329, ECO:0000312|HGNC:HGNC:8912} |
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Function | Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus (PubMed:11302691, PubMed:20959514, PubMed:28017329, PubMed:31522117). Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner (By similarity). Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (By similarity). |
Cellular Location | Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane |
Tissue Location | Widely expressed in different tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.
References
Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008)
Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008)
White,J.J., Genomics 11 (1), 228-230 (1991)
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