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PHB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - PHB Antibody (Center) AP2710C
    Confocal immunofluorescent analysis of PHB Antibody (Center)(Cat#AP2710c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
    detail
  • WB - PHB Antibody (Center) AP2710C
    Western blot analysis of PHB Antibody (Center) (Cat.#AP2710c) in Hela cell line lysates (35ug/lane). PHB (arrow) was detected using the purified Pab.
    detail
  • WB - PHB Antibody (Center) AP2710C
    Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (Center) (Cat.#AP2710c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
    detail
  • IHC-P - PHB Antibody (Center) AP2710C
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody (Center) (Cat. #AP2710c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - PHB Antibody (Center) AP2710C
    PHB Antibody (Center) (Cat. #AP2710c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, IF, FC, E
Primary Accession P35232
Other Accession P84173, Q3T165
Reactivity Human
Predicted Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 29804 Da
Antigen Region 89-117 aa
Additional Information
Gene ID 5245
Other Names Prohibitin, PHB
Target/Specificity This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHB1 {ECO:0000303|PubMed:28017329, ECO:0000312|HGNC:HGNC:8912}
Function Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus (PubMed:11302691, PubMed:20959514, PubMed:28017329, PubMed:31522117). Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner (By similarity). Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (By similarity).
Cellular Location Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane
Tissue Location Widely expressed in different tissues.
Research Areas
Citations (0)
citation

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Background

Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.

References

Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008)
Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008)
White,J.J., Genomics 11 (1), 228-230 (1991)

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$ 182.50
$ 70.00
Cat# AP2710C
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