TPST1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | O60507 |
Other Accession | Q3KR92, O70281, NP_003587 |
Reactivity | Human, Mouse |
Predicted | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 42188 Da |
Antigen Region | 336-366 aa |
Gene ID | 8460 |
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Other Names | Protein-tyrosine sulfotransferase 1, Tyrosylprotein sulfotransferase 1, TPST-1, TPST1 |
Target/Specificity | This TPST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-366 amino acids from the C-terminal region of human TPST1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TPST1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TPST1 |
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Function | Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate. |
Cellular Location | Golgi apparatus membrane; Single-pass type II membrane protein |
Tissue Location | Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
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Provided below are standard protocols that you may find useful for product applications.
Background
The ADP-ribosylation factor (Arf) family are highly conserved members of the Ras superfamily of regulatory GTP-binding proteins. Arf proteins participate in routing of intracellular proteins to and within the Golgi complex. Cellular functions include maintenance of organelle integrity, coat protein assembly, as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization.
References
Seibert, C., et al., Proc. Natl. Acad. Sci. U.S.A. 99(17):11031-11036 (2002).
Goettsch, S., et al., Biochem. Biophys. Res. Commun. 294(3):541-546 (2002).
Popovic, M., et al., Eur. J. Hum. Genet. 10(4):250-258 (2002).
Ouyang, Y.B., et al., J. Biol. Chem. 273(38):24770-24774 (1998).
Ouyang, Y., et al., Proc. Natl. Acad. Sci. U.S.A. 95(6):2896-2901 (1998).
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