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ART3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ART3 Antibody (N-term) AP2312a
    The anti-ART3 Pab (Cat. #AP2312a) is used in Western blot to detect ART3 in mouse brain tissue lysate.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q13508
Other Accession NP_001170
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43923 Da
Antigen Region 12-42 aa
Additional Information
Gene ID 419
Other Names Ecto-ADP-ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, Mono(ADP-ribosyl)transferase 3, NAD(P)(+)--arginine ADP-ribosyltransferase 3, ART3, TMART
Target/Specificity This ART3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human ART3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsART3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ART3
Synonyms TMART
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Testis specific.
Research Areas
Citations (0)
citation

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Background

Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Koch-Nolte, F., et al., Genomics 39(3):370-376 (1997).
Levy, I., et al., FEBS Lett. 382(3):276-280 (1996).

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$ 182.50
$ 70.00
Cat# AP2312a
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