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Neurofilament-L antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Neurofilament-L antibody AP22455a
    All lanes: Anti-Neurofilament-L antibody at 1:2000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 62 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P07196
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 61517 Da
Additional Information
Gene ID 4747
Other Names Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein, NEFL, NF68, NFL
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament-L antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEFL
Synonyms NF68, NFL
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Cellular Location Cell projection, axon {ECO:0000250|UniProtKB:P08551}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P08551}
Research Areas
Citations (0)
citation

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Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

References

Julien J.-P.,et al.Biochim. Biophys. Acta 909:10-20(1987).
Perez-Olle R.,et al.J. Cell Sci. 115:4937-4946(2002).
Nusbaum C.,et al.Nature 439:331-335(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Pospelov V.A.,et al.Cell Growth Differ. 5:187-196(1994).

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$ 325.00
$ 140.00
Cat# AP22455a
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