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PDHE1A(S232) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PDHE1A(S232) Antibody AP22387a
    All lanes: Anti-PDHE1A(S232) Antibody at 1:2000 dilution + PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 43 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - PDHE1A(S232) Antibody AP22387a
    All lanes : Anti-PDHE1A(S232) Antibody at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: RD whole cell lysate Lane 4: Mouse brain whole lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - PDHE1A(S232) Antibody AP22387a
    All lanes : Anti-PDHE1A(S232) Antibody at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08559
Other Accession A7MB35, Q8HXW9, P35486, P29804, P26284
Reactivity Human, Mouse, Rat
Predicted Bovine, Monkey, Pig
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 43296 Da
Additional Information
Gene ID 5160
Other Names Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, 1.2.4.1, PDHE1-A type I, PDHA1, PHE1A
Target/Specificity This PDHE1A(S232) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 206-237 amino acids from the human region of human PDHE1A(S232).
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDHE1A(S232) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDHA1
Synonyms PHE1A
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Tissue Location Ubiquitous.
Research Areas
Citations (0)
citation

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Background

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

References

Koike K.,et al.Gene 93:307-311(1990).
Ho L.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:5330-5334(1989).
Huh T.L.,et al.Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases.
Dahl H.-H.M.,et al.J. Biol. Chem. 262:7398-7403(1987).
Maragos C.,et al.J. Biol. Chem. 264:12294-12298(1989).

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$ 365.00
$ 140.00
Cat# AP22387a
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