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MUT Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - MUT Antibody (Center) AP22355c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling MUT with AP22355c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm mainly and mitochondrion staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
    detail
  • WB - MUT Antibody (Center) AP22355c
    All lanes : Anti-MUT Antibody (Center) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - MUT Antibody (Center) AP22355c
    All lanes : Anti-MUT Antibody (Center) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Mouse liver lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P22033
Other Accession Q5RFN2
Reactivity Human, Mouse
Predicted Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 83134 Da
Additional Information
Gene ID 4594
Other Names Methylmalonyl-CoA mutase, mitochondrial, MCM, 5.4.99.2, Methylmalonyl-CoA isomerase, MUT
Target/Specificity This MUT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 492-526 amino acids from the Central region of human MUT.
Dilution IF~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMUT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMUT (HGNC:7526)
Function Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.
Cellular Location Mitochondrion matrix. Mitochondrion. Cytoplasm
Research Areas
Citations (0)
citation

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Background

Involved in the degradation of several amino acids, odd- chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

References

Jansen R.,et al.Genomics 4:198-205(1989).
Nham S.U.,et al.Genomics 8:710-716(1990).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).

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$ 182.50
$ 70.00
Cat# AP22355c
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