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MUT Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - MUT Antibody (N-Term) AP22354a
    All lanes : Anti-MUT Antibody (N-Term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - MUT Antibody (N-Term) AP22354a
    All lanes : Anti-MUT Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - MUT Antibody (N-Term) AP22354a
    Immunohistochemical analysis of paraffin-embedded human testis tissue using AP22354a performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/250) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P-Leica, E
Primary Accession P22033
Other Accession Q9GK13, Q8HXX1, P16332, Q5RFN2
Reactivity Human, Mouse
Predicted Bovine, Monkey
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 83134 Da
Additional Information
Gene ID 4594
Other Names Methylmalonyl-CoA mutase, mitochondrial, MCM, 5.4.99.2, Methylmalonyl-CoA isomerase, MUT
Target/Specificity This MUT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-66 amino acids from the human region of human MUT.
Dilution WB~~1:2000
IHC-P-Leica~~1:250
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMUT Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMUT (HGNC:7526)
Function Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.
Cellular Location Mitochondrion matrix. Mitochondrion. Cytoplasm
Research Areas
Citations (0)
citation

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Background

Involved in the degradation of several amino acids, odd- chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

References

Jansen R.,et al.Genomics 4:198-205(1989).
Nham S.U.,et al.Genomics 8:710-716(1990).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).

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$ 365.00
$ 140.00
Cat# AP22354a
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