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IL1RAP Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IL1RAP Antibody (C-Term) AP22328a
    Anti-IL1RAP Antibody (C-Term) at 1:2000 dilution + Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - IL1RAP Antibody (C-Term) AP22328a
    AP22328a staining IL1RAP in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NPH3
Other Accession P59822
Reactivity Human, Mouse
Predicted Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 65418 Da
Additional Information
Gene ID 3556
Other Names Interleukin-1 receptor accessory protein, IL-1 receptor accessory protein, IL-1RAcP, Interleukin-1 receptor 3, IL-1R-3, IL-1R3, IL1RAP, C3orf13, IL1R3
Target/Specificity This IL1RAP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 484-518 amino acids of human IL1RAP.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL1RAP Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL1RAP
Synonyms C3orf13, IL1R3
Function Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable).
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 3]: Secreted.
Tissue Location Detected in liver, skin, placenta, thymus and lung. Isoform 4 is predominantly expressed in brain. Overexpressed on candidate chronic myeloid leukemia (CML) stem cells, hematopoietic stem cells and mononuclear cells of patients with acute myeloid leukemia (AML). Overexpressed in patients with chronic obstructive pulmonary disease (COPD). Expressed in T-helper 1 (Th1) and T-helper 2 (Th2) cell subsets (PubMed:10653850).
Research Areas
Citations (0)
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Background

Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1- dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non- signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand- bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

References

Huang J.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:12829-12832(1997).
Saito T.,et al.Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
Jensen L.E.,et al.J. Immunol. 164:5277-5286(2000).
Jensen L.E.,et al.Cell. Signal. 15:793-802(2003).
Lu H.L.,et al.Mol. Immunol. 45:1374-1384(2008).

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$ 182.50
$ 70.00
Cat# AP22328a
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