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HSD17B10 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - HSD17B10 Antibody (Center) AP22211c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HSD17B10 with AP22211c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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  • WB - HSD17B10 Antibody (Center) AP22211c
    All lanes : Anti-HSD17B10 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: rat brain lysate Lane 7: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - HSD17B10 Antibody (Center) AP22211c
    AP22211c staining HSD17B10 in human thyroid carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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  • FC - HSD17B10 Antibody (Center) AP22211c
    Overlay histogram showing Hela cells stained with AP22211c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22211c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, IF, E
Primary Accession Q99714
Other Accession O02691, O08756, O70351
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 26923 Da
Additional Information
Gene ID 3028
Other Names 3-hydroxyacyl-CoA dehydrogenase type-2, 1.1.1.35, 17-beta-hydroxysteroid dehydrogenase 10, 17-beta-HSD 10, 1.1.1.51, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 1.1.1.178, 3-hydroxyacyl-CoA dehydrogenase type II, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, Mitochondrial ribonuclease P protein 2, Mitochondrial RNase P protein 2, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH, HSD17B10, ERAB, HADH2, MRPP2, SCHAD, XH98G2
Target/Specificity This HSD17B10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 140-172 amino acids from the Central region of human HSD17B10.
Dilution IF~~1:25
WB~~1:2000
IHC-P~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSD17B10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSD17B10
Synonyms ERAB, HADH2, MRPP2, SCHAD, SDR5C1, XH98G
Function Mitochondrial dehydrogenase involved in pathways of fatty acid, branched-chain amino acid and steroid metabolism (PubMed:10600649, PubMed:12917011, PubMed:18996107, PubMed:19706438, PubMed:20077426, PubMed:25925575, PubMed:26950678, PubMed:28888424, PubMed:9553139). Acts as (S)-3-hydroxyacyl-CoA dehydrogenase in mitochondrial fatty acid beta-oxidation, a major degradation pathway of fatty acids. Catalyzes the third step in the beta-oxidation cycle, namely the reversible conversion of (S)-3-hydroxyacyl-CoA to 3- ketoacyl-CoA. Preferentially accepts straight medium- and short-chain acyl-CoA substrates with highest efficiency for (3S)-hydroxybutanoyl- CoA (PubMed:10600649, PubMed:12917011, PubMed:25925575, PubMed:26950678, PubMed:9553139). Acts as 3-hydroxy-2-methylbutyryl-CoA dehydrogenase in branched-chain amino acid catabolic pathway. Catalyzes the oxidation of 3-hydroxy-2-methylbutanoyl-CoA into 2-methyl-3- oxobutanoyl-CoA, a step in isoleucine degradation pathway (PubMed:18996107, PubMed:19706438, PubMed:20077426). Has hydroxysteroid dehydrogenase activity toward steroid hormones and bile acids. Catalyzes the oxidation of 3alpha-, 17beta-, 20beta- and 21- hydroxysteroids and 7alpha- and 7beta-hydroxy bile acids (PubMed:10600649, PubMed:12917011). Oxidizes allopregnanolone/brexanolone at the 3alpha-hydroxyl group, which is known to be critical for the activation of gamma-aminobutyric acid receptors (GABAARs) chloride channel (PubMed:19706438, PubMed:28888424). Has phospholipase C-like activity toward cardiolipin and its oxidized species. Likely oxidizes the 2'-hydroxyl in the head group of cardiolipin to form a ketone intermediate that undergoes nucleophilic attack by water and fragments into diacylglycerol, dihydroxyacetone and orthophosphate. Has higher affinity for cardiolipin with oxidized fatty acids and may degrade these species during the oxidative stress response to protect cells from apoptosis (PubMed:26338420). By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD) (PubMed:9338779). Essential for structural and functional integrity of mitochondria (PubMed:20077426).
Cellular Location Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid
Tissue Location Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD.
Research Areas
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Background

Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).

References

Yan S.D.,et al.Nature 389:689-695(1997).
Zhuchenko O.P.,et al.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Miller A.P.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8709-8714(1998).
He X.Y.,et al.J. Biol. Chem. 273:10741-10746(1998).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP22211c
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