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TK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TK1 Antibody (Center) AP22210c
    All lanes : Anti-TK1 Antibody (Center) at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - TK1 Antibody (Center) AP22210c
    AP22210c staining TK1 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - TK1 Antibody (Center) AP22210c
    Overlay histogram showing U-2 OS cells stained with AP22210c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22210c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P04183
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 25469 Da
Additional Information
Gene ID 7083
Other Names Thymidine kinase, cytosolic, 2.7.1.21, TK1
Target/Specificity This TK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 139-173 amino acids from the Central region of human TK1.
Dilution WB~~1:2000
IHC-P~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TK1 (HGNC:11830)
Function Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed:9575153). Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed:22385435). Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c- azido-3c-deoxythymidine (AZT) (PubMed:22385435).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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References

Bradshaw H.D. Jr.,et al.Mol. Cell. Biol. 4:2316-2320(1984).
Flemington E.,et al.Gene 52:267-277(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Kreidberg J.A.,et al.Mol. Cell. Biol. 6:2903-2909(1986).

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$ 365.00
$ 140.00
Cat# AP22210c
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