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EEF1A1P5 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - EEF1A1P5 Antibody (C-Term) AP22206b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EEF1A1P5 with AP22206b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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  • WB - EEF1A1P5 Antibody (C-Term) AP22206b
    All lanes : Anti-EEF1A1P5 Antibody (C-Term) at 1:1000-1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: 293 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • FC - EEF1A1P5 Antibody (C-Term) AP22206b
    Overlay histogram showing HepG2 cells stained with AP22206b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22206b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IF, E
Primary Accession Q5VTE0
Other Accession P68103, P62629, Q66RN5, A2Q0Z0, P68104, P10126, Q5R1X2, Q5R4R8, P68105, P62630, Q90835
Reactivity Human
Predicted Bovine, Hamster, Horse, Mouse, Rabbit, Rat, Chicken
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 50185 Da
Antigen Region 430-462 aa
Additional Information
Other Names Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, eEF1A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5, EEF1A1P5, EEF1AL3
Target/Specificity This EEF1A1P5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 430-462 amino acids from human EEF1A1P5.
Dilution IF~~1:25
WB~~1:1000-1:2000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1A1P5 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1A1P5
Synonyms EEF1AL3
Function This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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Background

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

References

Humphray S.J.,et al.Nature 429:369-374(2004).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
Li W.B.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Lund A.,et al.Genomics 36:359-361(1996).

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$ 365.00
$ 140.00
Cat# AP22206b
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