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ARF4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ARF4 Antibody (Center) AP22186c
    All lanes : Anti-ARF4 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Caki-1 whole cell lysate Lane 4: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P18085
Other Accession Q5RCF1
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 20511 Da
Additional Information
Gene ID 378
Other Names ADP-ribosylation factor 4, ARF4, ARF2
Target/Specificity This ARF4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 72-106 amino acids from the Central region of human ARF4.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARF4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARF4
Synonyms ARF2
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation (PubMed:25673879).
Cellular Location Golgi apparatus. Membrane; Lipid-anchor
Research Areas
Citations (0)
citation

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Background

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

References

Monaco L.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210(1990).
Kahn R.A.,et al.J. Biol. Chem. 266:2606-2614(1991).
Lebeda R.A.,et al.Gene 237:209-214(1999).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 140.00
Cat# AP22186c
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