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TFF1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - TFF1 Antibody (Center) AP22076c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling TFF1 with AP22076c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing secreted staining on MCF-7 cell line. The nuclear counter stain is DAPI (blue).
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  • WB - TFF1 Antibody (Center) AP22076c
    All lanes : Anti-TFF1 Antibody (Center) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: T47D whole cell lysate Lane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 9 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P04155
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 9150 Da
Additional Information
Gene ID 7031
Other Names Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, TFF1, BCEI, PS2
Target/Specificity This TFF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the Central region of human TFF1.
Dilution IF~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFF1
Synonyms BCEI, PS2
Function Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
Cellular Location Secreted
Tissue Location Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level) Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).
Research Areas
Citations (0)
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Background

Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

References

Jakowlew S.B.,et al.Nucleic Acids Res. 12:2861-2878(1984).
Prud'Homme J.-F.,et al.DNA 4:11-21(1985).
Jeltsch J.-M.,et al.Nucleic Acids Res. 15:1401-1414(1987).
Takahashi H.,et al.FEBS Lett. 261:283-286(1990).
Mori K.,et al.J. Biochem. 107:73-76(1990).

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$ 365.00
$ 140.00
Cat# AP22076c
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