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GBGT1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GBGT1 Antibody (Center) AP22026c
    All lanes : Anti-GBGT1 Antibody (Center) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: SK-OV-3 whole cell lysate Lane 4: A2780 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8N5D6
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 40127 Da
Antigen Region 154-185 aa
Additional Information
Gene ID 26301
Other Names Globoside alpha-1, 3-N-acetylgalactosaminyltransferase 1, 2.4.1.-, Forssman glycolipid synthase-like protein, GBGT1
Target/Specificity This GBGT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 154-185 amino acids from the Central region of human GBGT1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGBGT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GBGT1 (HGNC:20460)
Function Has lost the ability to synthesize Forssman glycolipid antigen (FORS1/FG) (PubMed:10506200). Might have acquired an alternative function in glycosphingolipid metabolism, but it remains to be established. It appears to have drifted more slowly than confirmed pseudogenes in the glycosyltransferase 6 family, suggesting that it has remained under evolutionary pressure.
Cellular Location Golgi apparatus membrane; Single- pass type II membrane protein
Tissue Location Widely expressed. Expressed at higher level in placenta, ovary and peripheral blood leukocyte, whereas it is weakly expressed in liver, thymus, and testis (PubMed:10506200). Expressed in bone marrow erythroid cells (PubMed:23255552)
Research Areas
Citations (0)
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Background

Catalyzes the formation of some glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to some substrate. Glycolipids probably serve for adherence of some pathogens.

References

Xu H.,et al.J. Biol. Chem. 274:29390-29398(1999).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP22026c
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