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TPM3 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TPM3 Antibody (C-Term) AP21987b
    All lanes : Anti-TPM3 Antibody (C-Term) at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P06753
Other Accession Q5KR49, P04268, P58773, P13104, P09493, P84335, P58771, P42639, P58772, P13105, P04692, Q01173, Q5KR47, A1XQV4
Reactivity Human
Predicted Bovine, Chicken, Zebrafish, Mouse, Pig, Rabbit, Rat, Xenopus
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 32950 Da
Additional Information
Gene ID 7170
Other Names Tropomyosin alpha-3 chain, Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, hTM5, TPM3
Target/Specificity This TPM3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 245-276 amino acids from the human region of human TPM3.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPM3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPM3
Function Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)
citation

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Background

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

References

Reinach F.C.,et al.Nature 322:648-650(1986).
McLeod A.R.,et al.Nucleic Acids Res. 14:8413-8426(1986).
Clayton L.,et al.J. Mol. Biol. 201:507-515(1988).
Lin J.J.-C.,et al.Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).

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$ 140.00
Cat# AP21987b
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